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Pelicluster CD41, 1ml
| Monoclonal/ Polyclonal: | Monoclonal |
| Clone: | CLB-tromb/7, 6C9 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Application: | Flow cytometry (FC) |
| Application notes: | The monoclonal mouse antibody is a useful marker for studies of megakaryoblasts and megakaryoblastic leukaemias. Method: Indirect immunofluorescence staining with analysis by flow cytometry or fluorescence microscopy. |
| Conjugation Type: | Conjugated |
| Conjugate: | FITC |
| Conjugation note: | FITC conjugated Goat anti-Mouse immunoglobulin antiserum, solid phase adsorbed with human immunoglobulins or comparable product. Remove aggregates by centrifugation at 1000 x g for 10 minutes. Dilute as mentioned in the procedure. |
| Purified: | YES |
| Purification note: | Culture supernatant purified by affinity chromatography. |
| Buffer: | Wash- and dilution buffer for mononuclear cells,(PBS/BSA): Phosphate Buffered Saline containing 0.2% BSA (w/v). Wash- and dilution buffer for platelets, (Seq). Sequestrine buffer, storage 1 month at 2-8°C. 10 x stock solution, dissolve in 5 |
| UNSPSC code: | 41110000 |
Pelicluster CD41, 1ml
Pelicluster CD41, 1ml
This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human platelets. The antibody was submitted to CD41a in the Third, Fourth and Sixth International Workshop on Human Leukocyte Differentiation Antigens. Source: Culture supernatant purified by affinity chromatography.
Apparent molecular mass under reducing conditions: GPIIIa:110 kDa; GPIIb alpha chain:125 kDa, beta chain:
25 kDa.
The monoclonal mouse antibody reacts with megakaryocytes, platelets and recognize the intact thrombocyte
GPIIb/IIIa complex, i.e. it does not bind to dissociated GPIIb or GPIIIa. The monoclonal mouse antibody does
not bind or reduced to thrombocytes from patients with Glanzmann’s thrombasthenia and do not recognize
the vitronectin receptor, which contains GPIIa as a beta subunit, cf. CD61 (1).
Alternative names: