Interferon-gamma (IFNG) is a potent multifunctional cytokine secreted primarily by activated lymphocytes such as CD4 T helper type 1 (Th1) cells and CD8 cytotoxic T cells, NK cells, B cells, and antigen-presenting cells.. Originally characterized based on anti-viral activities, IFN-gamma also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-gamma can upregulate MHC class I and II antigen expression by antigen-presenting cells. IFNG expression is induced by mitogens and cytokines. The downstream target genes of IFNG signaling pathway regulate several biological functions, including cell cycle, apoptosis, and inflammation. In adaptive immunity, IFNG directly regulates the differentiation, activation, and homeostasis of Th1 cells; inhibits Th2 cell development; promotes regulatory T cell development and natural killer cell activity. This antibody recognizes a human interferon protein of 20-25kDa, and both recombinant and native human IFNG. It is specific to

Tryptase [EP259]

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are stored in mast cell secretory granules and basophils. These enzymes are released into the extracellular environment, and are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. There are two separate genes: alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. Anti-tryptase is a good marker for mast cells, basophils, and their derivatives. Mastocytosis is a term collectively used for a group of disorders in which there is abnormal accumulation of mast cells in one or multiple organs. Anti-tryptase, combined with anti-CD2, anti-CD25, and anti-CD117, can be useful in the differential diagnosis of reactive mast cell hyperplasia, myelogenous neoplasms, mast cell leukemia, and mastocytosis.

Tryptase [EP259]