The dual-color FluoroSpot assay is a highly sensitive technique to monitor ex vivo cellular immune responses at single cell level. This 2-plate format kit is supplied with all necessary reagents, such as coating and detection antibodies, fluorescent-labeled conjugate, buffers and 96-well PVDF-membrane bottomed plates, to perform 192 FluoroSpot determinations. A detailed manual is enclosed. A fluorescent microscope or automated ELISPOT reader with a multicolor optical filter set for FITC and R-PE is required. (Shipping cost: €30.00; Handling cost €30.00 - €50.00)