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Monoclonal antibody to rat IFN-gamma (clone DB-12)
| Product group: | Primary |
| Monoclonal/ Polyclonal: | Monoclonal |
| Clone: | DB-12 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Application: | ELISA,Enzyme-linked immunosorbent spot (ELISPOT),Immunohistochemistry on frozen sections (IHC-Fs),Western Blot (WB) |
| Conjugation Type: | Unconjugated |
| Purified: | YES |
| Purification note: | Ion exchange chromatography. |
| Does not react with: | Mouse, Rodent, Human |
| Research area: | Cytokine, Immunology, Animal targets, T cells |
| General notes: | Availablitiy : Delivery usually within 8 business days..Non Confirming product: Replace the product at no costs..Restriction: This product is for research use only and is not approved for use in diagnostic or therapeutic procedures. The product is guaranteed for 1 year from date of receipt..Material Safety datasheet: https://www.ucytech.com/sites/default/files/manuals/MSDS%20Antibody_0.pdf |
| Form: | Lyophilized |
| Buffer: | Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose |
| Storage: | The lyophilized product is stable for at least 1 year at 4°C. 0.5 kg |
| UNSPSC code: |
The mouse anti-rat IFN-γ monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-γ antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity and it has shown not to bind with either mouse IFN-γ nor human IFN-γ. Further, the DB-12 antibody is highly effective as coating antibody in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody (cat no. CT035) for detection. Also the reverse combination in which the DB-1 antibody (cat. no. CT032) functions as coating antibody and biotinylated DB-12 antibody (cat. no. CT033) as detector can be used in an ELISA system. The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining. (Shipping cost: €30.00; Handling cost €30.00 - €50.00)
Monoclonal antibody to rat IFN-gamma (clone DB-12)
The mouse anti-rat IFN-γ monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-γ antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity and it has shown not to bind with either mouse IFN-γ nor human IFN-γ. Further, the DB-12 antibody is highly effective as coating antibody in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody (cat no. CT035) for detection. Also the reverse combination in which the DB-1 antibody (cat. no. CT032) functions as coating antibody and biotinylated DB-12 antibody (cat. no. CT033) as detector can be used in an ELISA system. The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining.
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