You are here
Products
Monoclonal antibody to rat IFN-gamma (clone DB-1)
| Product group: | Primary |
| Monoclonal/ Polyclonal: | Monoclonal |
| Clone: | DB-1 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Application: | ELISA,Enzyme-linked immunosorbent spot (ELISPOT),Immunohistochemistry on frozen sections (IHC-Fs),Neutralization (Neut),Western Blot (WB) |
| Conjugation Type: | Unconjugated |
| Purified: | YES |
| Purification note: | Ion exchange chromatography. |
| Does not react with: | rodent IFN-α, rodent IFN-β, human IFNs |
| Research area: | Cytokine, Immunology, Animal targets, T cells |
| General notes: | Availablitiy : Delivery usually within 8 business days..Non Confirming product: Replace the product at no costs..Restriction: This product is for research use only and is not approved for use in diagnostic or therapeutic procedures. The product is guaranteed for 1 year from date of receipt..Material Safety datasheet: https://www.ucytech.com/sites/default/files/manuals/MSDS%20Antibody_0.pdf |
| Form: | Lyophilized |
| Buffer: | Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose |
| Storage: | The lyophilized product is stable for at least 1 year at 4°C. 0.5 kg |
| UNSPSC code: |
The mouse anti-rat IFN-γ monoclonal antibody (clone DB-1) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-γ antibody is of the IgG1 subtype and neutralizes both natural and recombinant rat IFN-γ and mouse IFN-γ in vitro and in vivo. In Western blot analysis the DB-1 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity. Clone DB-1 has shown not to bind with rat IFN-α and IFN-β nor human IFNs. Further, biotinyated DB-1 antibody (cat. no. CT035) is highly effective as detector antibody in both sandwich ELISA and ELISPOT assays using DB-12 (cat no. CT052) as coating antibody. Also the reverse combination in which the DB-1 antibody functions as coating antibody and biotinylated DB-12 antibody (cat. no. CT033) or biotinylated polyclonal antibody to rat IFN-γ as detector antibody can be used in an ELISA and ELISPOT systems. The DB-1 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular stain
Monoclonal antibody to rat IFN-gamma (clone DB-1)
The mouse anti-rat IFN-γ monoclonal antibody (clone DB-1) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-γ antibody is of the IgG1 subtype and neutralizes both natural and recombinant rat IFN-γ and mouse IFN-γ in vitro and in vivo. In Western blot analysis the DB-1 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity. Clone DB-1 has shown not to bind with rat IFN-α and IFN-β nor human IFNs. Further, biotinyated DB-1 antibody (cat. no. CT035) is highly effective as detector antibody in both sandwich ELISA and ELISPOT assays using DB-12 (cat no. CT052) as coating antibody. Also the reverse combination in which the DB-1 antibody functions as coating antibody and biotinylated DB-12 antibody (cat. no. CT033) or biotinylated polyclonal antibody to rat IFN-γ as detector antibody can be used in an ELISA and ELISPOT systems. The DB-1 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining. Other available formats: biotin labeled (cat. no. CT035) and FITC labeled (cat. no. CT034).
Alternative names: